Effects of Caffeine and 5-Caffeoylquinic Acid on Blood Cell In Vitro Cytokine Production in Response to Lipopolysaccharide Stimulation

Previous in vitro studies the have suggested anti-inflammatory properties of caffeine. Coffee is also rich phenolic acids with several potential health benefits (chlorogenic acids), mainly 5-caffeoylquinic acid (5-CQA). The aim present study was to determine effect caffeine and 5-CQA, using concentrations comparable typical human consumption, on LPS-induced cytokine production whole blood cultures. Whole samples (n = 10) were stimulated for 24 h LPS presence or 5-CQA. various cytokines, malondialdehyde (MDA) adenosine-3′,5′-cyclic monophosphate (cAMP) determined culture media. Significant effects found only TNF-α (p 0.014) cAMP 0.001). However, highest concentration (50 μM) induced lower 0.016) higher 0.006) levels comparison control. 5-CQA influenced IL-6 0.002), values (2 μM: p 0.012; 20 < Neither 0.227) nor 0.259) MDA concentrations. In conclusion, both exerted limited effects. For caffeine, these attained high physiological supraphysiological needed.